Automates culture medium replacement at low cost!
Suitable for applications where constant and frequent medium replacement is needed (e.g. iPS cell culture)
1500,- €
* All prices are net, plus VAT and shipping
Features
Automatic medium replacement
The culture medium is replaced automatically at a specified interval (3, 6, 12, or 24 hours).
Can be used in an incubator
The system can operate continuously for up to 7 days with batteries, and be placed in an incubator without any wiring.
Accommodates a standard 6-well plate
A standard 6-well plate can be used, which reduces running cost and minimizes environmental changes resulting from introducing the system.
Observable on a microscope
The 6-well plate can remain in the system and be placed on a microscope stage when you observe the cells, which also reduces environmental changes.
Disposable
All system components that are exposed to the medium can be replaced.
Application Examples
Case 1. hiPSC (human induced pluripotent stem cells)
Colony growth was successfully observed for 4 days after passage (engraftment), during which the culture medium was automatically replaced by our system.
[Experiment Condition]
･Cells: 253G1 Human iPS cells (NHDF reprogrammed by 3 factors; Oct3/4, Sox2, Klf4)
･Feeder Cells: Mitomycin C treated SNL76/7 cells
･Medium: DMEM-12 (including KSR, NEAA, PSG, 2-ME, bFGF)
Case 2. Embryoid bodies (EB) of mouse iPS cells
iPS cells were cultured in Knockout D-MEM based myocardial differentiation medium using our system, and pulsation of differentiation-inducted cardiomyocytes derived from mouse iPS cells was observed.
Specifications
| Model Number | CEME-0102 |
|---|---|
| Outer Dimensions | W194×D228×H140(mm) |
| Product Weight | Approx. 500g (Medium weight not included) |
| Power Supply | AA alkaline battery *2 |
| Applicable Culture Vessel | 6 well plate *1 |
| Initilally Required Medium Volume (per well) | 3ml *2 |
| Volume Replaced (per well) | 2.7ml*2 |
| Medium Replacement Interval | 3h / 6h / 12h / 24h |
| Wetted Part Material | Silicone, SUS304, PP |
| Bottle Capacity | 125ml |
| Price |
*1 Recommened well plate: Thermo Fisher Scientific Inc.: NUNC #140675, Sumitomo Bakelite Co., Ltd. MS-80060
*2 Volume may slightly vary depending on the type of well plate.